Fig. 2: 3D image recordings of CRY2-olig proteins at different activation schemes. | Communications Biology

Fig. 2: 3D image recordings of CRY2-olig proteins at different activation schemes.

From: Optogenetic manipulation of cell migration with high spatiotemporal resolution using lattice lightsheet microscopy

Fig. 2

a The schematic for various activations: Bessel fan, shifted Bessel fan, and single Bessel beam. The activation beam illuminates on the Y’Z’ plane at an angle of 32.80. Bottom: The corresponding timing diagrams for the SLM patterns with laser 488 nm Bessel beam (blue) and 560 nm lattice beam (green) illumination synchronized with sample piezo waveforms (black). b The time-lapse images of optical induced clustering of CRY2olig-mRuby3 in XY’ Y’Z’ XZ’ planes stimulated by Bessel fan and shifted Bessel fan activation. The depth of the oligomerization is color-coded, where the process of oligomerization occurs only at the basal membrane by the shifted Bessel beam activation. c The center part of the cell marked by the white cross is illuminated by the single Bessel beam. Oligomerization occurs near the activation site. Scale bar 10 μm.

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