Fig. 4: The dynamics of the cellular membranes upon sub-cellular activation. | Communications Biology

Fig. 4: The dynamics of the cellular membranes upon sub-cellular activation.

From: Optogenetic manipulation of cell migration with high spatiotemporal resolution using lattice lightsheet microscopy

Fig. 4

a The cells expressing F-tractin-mCherry-p2a-CRY2mCFPiSH-p2a-CIBNEYFP-CAAX are illuminated by a single Bessel beam activation. The cross represents the activation site. To demonstrate the dynamic behavior of filamentous actin, intensity contours were plotted before and after the stimulation. At region 1, the distance of the membrane edge along the white arrow is shown in the inset. Different color represents different time points after stimulation. The top images are XY orthographic view, and the bottom images are XZ view with 32.8° rotation on X-axis. The gray images are the original images. b The Bessel fan was used to stimulate the endothelial growth factor receptor (EGFR)-CRY2olig-mApplex3-expressing cells at the trailing edge. The images in the center row are the whole-cell images at different times post-stimulation. The stimulation areas are marked as cyan. The solid arrow indicates the original trailing edge, and the hollow arrow indicates the original leading edge. Images on the top and bottom rows are enlarged views of the dashed box area in the middle row. Different colors represent different time points. Scale bar 10 μm.

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