Fig. 5: The time-dependent trajectories of the cell in the guided migration experiment.

The cell expressing EGFR-CRY2olig-mApplex3 was stimulated by a 1 nW Bessel fan. Different colors represent different time points. The individual LLSM 3D rendering images of the cell at different times, and the stimulated areas are depicted at the bottom. The volume changes of the cell during the cell-guided migration are plotted in the inset. The interval between each stimulation and image is about 1 min. The entire experiment lasted 392 min. Scale bar 20 μm.