Fig. 4: Accumulation of antibiotics in PAO1 and PAO509. | Communications Biology

Fig. 4: Accumulation of antibiotics in PAO1 and PAO509.

From: The membrane-active polyaminoisoprenyl compound NV716 re-sensitizes Pseudomonas aeruginosa to antibiotics and reduces bacterial virulence

Fig. 4

Ciprofloxacin or rifampicin analog NV1532 were used alone (a, d) or combined with potentiators in PAO1 (b, e) or PAO509 (c, f). Potentiators were added at the indicated concentrations (alexidine [ALE]; polymyxin B nonapeptide [PMBN]; colistin [CST]). All data are mean ± SEM (triplicates from three independent experiments). Statistical analysis: Left-panels: Two-tail Student’s t test for comparison of antibiotics alone in PAO1 and PAO509; other panels: one-way ANOVA with Dunnett’s post-hoc test for comparison of antibiotics alone and combined with each potentiator: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Correlation between the accumulation of ciprofloxacin (g) or NV1532 (h) in bacteria and the outer membrane permeability as assessed by the fluorescence signal of NPN (see Fig. 3f–i). The Pearson correlation coefficient r and the p values are shown on each graph. The plain and dotted lines correspond to the linear regression with its 95% confidence interval.

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