Fig. 6: Transcriptomic analysis of PAO1 after serial passages with selected potentiators. | Communications Biology

Fig. 6: Transcriptomic analysis of PAO1 after serial passages with selected potentiators.

From: The membrane-active polyaminoisoprenyl compound NV716 re-sensitizes Pseudomonas aeruginosa to antibiotics and reduces bacterial virulence

Fig. 6

a Venn diagrams showing the commonly differentially expressed genes in PAO1 after 52 passages of culture in the absence or in the presence of NV716, colistin (CST) or alexidine (ALE) at ½ MIC. Pathways for intersecting genes that are relevant for this study are depicted in boxes. b, c GO term enrichment analysis of up- and downregulated genes, respectively, highlighting the biological functions that are exclusively regulated by a single potentiator or shared by two or three of them (A, red: NV716, B, purple: colistin, C, black: alexidine). The color gradient represents the value of the fold-change enrichment. All processes shown in these panels are significant (FDR < 0.05). d Relative expression of genes involved in the regulation of virulence or in LPS modifications in PAO1 after 6 h of incubation in the absence (Ctrl, normalized to 1) of or in presence of potentiators at 38 µM (PAβN) or ½ MIC (other molecules). Statistical analysis: a: the number of up- or downregulated genes is higher than expected by chance (higher than 97.5% of permutated data) for those up- or downregulated by the three potentiators or by NV716 and alexidine. d One-way ANOVA with Dunnett’s post-hoc test (*p < 0.05; **p < 0.01).

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