Fig. 7: Assays validating transcriptomic analyses, performed for the parental strain or the final isolate (passage 52) exposed to each potentiator at ½ MIC.

Motility assays (a swarming; b swimming; c twitching); scale bar: 2 cm; biofilm formation assessed by biomass quantification (d) and virulence factors secretion (e–g). In these panels, all data are expressed in percentage of the untreated control. Data are means of triplicates from 3 to 4 experiments. Statistical analysis: one-way ANOVA with Dunnett’s post-hoc test (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001).