Fig. 9: Confocal images of biofilms of PAO1 exposed to antibiotics and potentiators. | Communications Biology

Fig. 9: Confocal images of biofilms of PAO1 exposed to antibiotics and potentiators.

From: The membrane-active polyaminoisoprenyl compound NV716 re-sensitizes Pseudomonas aeruginosa to antibiotics and reduces bacterial virulence

Fig. 9

Potentiators were used in the conditions described in Fig. 8 (alexidine [ALE, 12 µM], polymyxin B nonapeptide [PMBN, 30 µM], colistin [CST, 1 µM]; NV716 50 µM). a Biofilms incubated with NV1532 (fluorescent derivative of rifampicin) at 100 mg/L alone or with potentiators, with fluorescence of NV1532 recorded in the green channel and fluorescence of CTC in the red channel. b Biofilms incubated with ciprofloxacin at 50 mg/L alone or with potentiators, with fluorescence of SYTO-9 recorded in the green channel and fluorescence of propidium iodide (PI) in the red channel. Scale bar: 30 µm. c, d quantitative analysis of the data from panel a, showing the fluorescence of NV1532 or of CTC, respectively, in percentage of the value measured at the top of the biofilm. e, f quantitative analysis of the data from panel b, showing the fluorescence of SYTO-9 or of PI, respectively, in percentage of the value measured at the top of the biofilm.

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