Fig. 3: PSMβ1 protects neutrophils from the inhibitory effect of PSMα3 on the neutrophil response to PMA.
From: Phenol-soluble modulin α and β display divergent roles in mice with staphylococcal septic arthritis
a NADPH oxidase–mediated superoxide anion release by neutrophils was measured by isoluminol-amplified chemiluminescence. The cells were preincubated at 37 °C for 5 min before being challenged with the first agonist (arrow to the left) followed by a second stimulus (PMA, 50 nM) once the first response had returned to baseline. Shown is one representative trace out of 10 individual experiments for neutrophils stimulated with PSMβ1 (500 nM), PSMα3 (500 nM), mixture of PSMα3 and PSMβ1 (500 + 500 nM) or buffer control and followed by PMA stimulation. b The peak O2− release by neutrophils stimulated with PMA following stimulation of PSMβ1 (500 nM), PSMα3 (500 nM), mixture of PSMα3 and PSMβ1 (500 + 500 nM) or buffer control was compared in 10 experiments. Statistical comparison was done using paired t test, with data expressed as mean ± standard error of the mean b. **P < 0.01; ***P < 0.001; ****P < 0.0001.
