Fig. 5: AdSpike has little effect on heart damage repair after myocardial I/R on hACE2 mice in vivo.

a Antibody tilters of SARS-CoV-2 spike receptor-binding domain (RBD) in the mouse serum collected at 4 weeks after injection of AdSpike at various doses. n = 7 for each group. b Representative immunostaining analysis of the ACE2⁺ cardiomyocytes with quantification of the mean fluorescence intensity in the heart at 4 weeks post-vaccination. n = 6 for each group. Scale bar, 20 μm. c Ejection fraction and fractional shortening of hACE2 mice heart after I/R measured by echocardiography at various time points post-I/R measured by echocardiography. D, day; W, week. n = 7 for each group. d Masson-Trichrome staining of heart cross sections of hACE2 mice 4 weeks post-I/R (left) with quantification of scar size (right). n = 7 for each group. Scale bar, 1 mm. e Quantification of heart weight (HW) relative to body weight (BW) of hACE2 mice at 4 weeks post-I/R. n = 7 for each group. f Representative and quantitative immunostaining analysis of TUNEL⁺/cTnT⁺ cardiomyocytes, CD31⁺ capillaries, and α-SMA⁺ vessels in the border zones of infarcted hACE2 mouse hearts 4 weeks post-I/R. n = 5–6 for each group. Scale bar, 50 μm g Representative immunostaining analysis of the ACE2⁺ cardiomyocytes with quantification of the mean fluorescence intensity in border zone (BZ) or remote zone (RZ) at 24 hours or 4 weeks post-I/R. n = 9–10 for each group. Scale bar, 10 μm. h Representative and quantitative immunostaining analysis of CD45⁺ cells in border zone (BZ) or remote zone (RZ) at 24 hours or 4 weeks post-I/R. n = 6 for each group. Scale bar, 10 μm. i Representative hematoxylin-eosin staining analysis of border zone (BZ) or remote zone (RZ) at 24 hours or 4 weeks post-I/R. Scale bar, 100 μm. Statistical significance was assessed using one-way ANOVA with Turkey post-test (a, b, d–f) or 2-way ANOVA with Turkey post-test (c, g and h). Data are presented as mean ± SEM; n.s., not significant.