Fig. 7: Inhibiting astrocytic activity with clozapine in GFAP-DREADD (Gi) expressing mice blocked cocaine-induced vasoconstriction.
From: Cocaine’s cerebrovascular vasoconstriction is associated with astrocytic Ca2+ increase in mice
Cocaine induced \(\Delta {F/F}_{{{{\rm{Ca}}}^{2+}}{\mbox{-}{{{\rm{G(D)}}}}}}\) (a, b), HbT (c, d), vessel diameter size (e, f), and HbO2 (g, h) changes as function of time before and after astrocytic ΔCa2+ inhibition by clozapine. i–l Quantification analysis of average efficiency in \(\Delta {F/F}_{{{{\rm{Ca}}}^{2+}}{\mbox{-}{{{\rm{G(D)}}}}}}\), vessels size, hemodynamic response respectively before DREADD(Gi) activation (Gray shadow in a, c, e, g) and after DREADD(Gi) activation by clozapine (Pink shadow in b, d, f, h). Meanwhile, yellow shadow indicates time periods with significant differences compared to baseline (p < 0.05), and green shadow indicate no-significant differences (p > 0.05). All error bars are presented as means ± SEM.
