Fig. 2: OT-hM3Dq-mCherry was functioning in the transgenic rats.
From: Endogenous oxytocin exerts anti-nociceptive and anti-inflammatory effects in rats
Endogenous mCherry, Fos-ir and merged images of the SON (a) and PVN (b) at 120 min after the s.c. injection of Saline or CNO (1 mg kg−1). c Percentage of Fos-ir neurons in mCherry neurons in the SON and PVN (n = 10–12 slices from 5 to 6 rats, each). **P < 0.01 vs. Saline. d The PVN was anatomically divided into magnocellular PVN (mPVN) and dorsal parvocellular PVN (dPVN). e Percentage of Fos-ir neurons in the mPVN and dPVN (n = 10–12 slices from 5 to 6 rats, each). **P < 0.01 vs. Saline. f Confocal images of endogenous mCherry (red), OT-ir (blue), Fos-ir (green) and merged images of a single OTPVN neuron at 120 min after the s.c. injection of CNO. White arrow heads, dendrite of OTPVN neuron. g The serum OT concentration at 0, 10, 30, 60, 120, and 180 min after the s.c. injection of Saline or CNO (n = 6–7 rats in each group at each time point). *P < 0.05; **P < 0.01 vs. Saline at the same time point. #P < 0.05; ##P < 0.01 vs. CNO at 0 min. h In situ hybridization (ISH) histochemistry of oxytocin (OXT) in the SON and PVN. i Gene expression of OXT in the SON, mPVN, and dPVN at 0, 30, 60, 120, and 180 min after the s.c. injection of Saline or CNO (n = 12 slices from 6 rats, each). **P < 0.01 vs. Saline at the same time point. ##P < 0.01 vs. CNO at 0 min. Scale bars in (a, b, d, h, 200 μm and 50 μm (in magnified images). Scale bars in (f), 10 μm. See also Supplementary Figs. 1, 2.
