Fig. 1: Characterization of SARS-CoV-2 nsp8.
a Gel filtration analysis of nsp8. b Molecular weight determination of nsp8 from two eluted peaks using DLS. c Hydrodynamic radius of nsp8 dimers and tetramers determined by DLS. Data measured by DLS are representative of three independent experiments. Error bars correspond to standard deviation. Protein was dissolved in a buffer of 20 mM HEPES pH 7.4, NaCl (125, 250, or 500 mM). d Radius of gyration (Rg) of nsp8 dimers and tetramers in buffers containing different concentrations of NaCl. The Rg quantities were obtained from model fitting to the SANS spectra of corresponding solutions as shown in e, error bars correspond to SANS fitting error. f Melting and g aggregation temperature of nsp8 dimers and tetramers. Data were representative of three independent experiments. Error bars correspond to standard deviation. Protein was dissolved in a buffer of 20 mM HEPES pH 7.4 and NaCl (150, 250, or 500 mM). Source data for (b–d, f, g) are available in Supplementary Data 1.
