Fig. 3: RNA modulates phase separation behaviors of nsp8 tetramer. | Communications Biology

Fig. 3: RNA modulates phase separation behaviors of nsp8 tetramer.

From: Multiscale characterization reveals oligomerization dependent phase separation of primer-independent RNA polymerase nsp8 from SARS-CoV-2

Fig. 3

a RNA induces LLPS of nsp8 tetramer in a sequence and length-dependent manner. Before imaging, nsp8 tetramer at 0.25 mg/mL was labeled with RED-tris-NTA and mixed with RNA in a buffer of 20 mM HEPES pH 7.4, 50 mM NaCl. b Co-localization of nsp8 tetramer and ssRNA R12 in liquid-like droplets. Before imaging, 0.25 mg/mL RED-tris-NTA labeled nsp8 tetramer was mixed with 75 μM 6-FAM labeled R12 in a buffer of 20 mM HEPES pH 7.4, 50 mM NaCl.

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