Fig. 5: Importance of houses and nesting material for expenditure, respiratory exchange ratio, food intake, water intake, and activity level in mid-old normal-weight and DIO male mice. | Communications Biology

Fig. 5: Importance of houses and nesting material for expenditure, respiratory exchange ratio, food intake, water intake, and activity level in mid-old normal-weight and DIO male mice.

From: Housing-temperature reveals energy intake counter-balances energy expenditure in normal-weight, but not diet-induced obese, male mice

Fig. 5

Data from mice housed hide and nesting material (dark blue), with house but without nesting material (light blue), and with house and with nesting material (orange). a, c, e, and g Energy expenditure (EE,kcal/h) at housing at 22, 25, 27.5, and 30 °C, b, d, f, and h average EE (kcal/h). i–p Data from mice housed at 22 °C: i Respiratory exchange ratio (RER, VCO2/VO2), j Average RER(VCO2/VO2), k cumulative food intake (g), l average food intake (g/24 h), m Cumulative water intake (mL), n average water intake AUCs (mL/24 h), o cumulative activity (m), p average activity level (m/24 h). Data are presented as means + SEM and dark phase (1800-0600h) is indicated with grey boxes. Dots in bar graphs indicate individual mice. Statistical significance was tested by Oneway-ANOVA for repeated measurements followed by Tukey multiple comparison test. *P < 0.05, **P < 0.01. Averages were calculated over the entire experimental period (0–72 h). n = 7.

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