Fig. 2: DHA enhances the bactericidal efficacy of colistin without affecting bacterial growth. | Communications Biology

Fig. 2: DHA enhances the bactericidal efficacy of colistin without affecting bacterial growth.

From: Commercialized artemisinin derivatives combined with colistin protect against critical Gram-negative bacterial infection

Fig. 2

a–d Growth curves for E. coli W3110 (pUC19-mcr-1) (a), E. coli ZJ487 (b), K. pneumoniae ZJ05 (c) and S. typhimurium HYM2 (d) cultured in the presence of various concentrations of DHA (0, 16–128 µg/mL). e–h Time-killing curves for DHA, colistin, combination and control treatment against E. coli W3110 (pUC19-mcr-1) (e), E. coli ZJ487 (f), K. pneumoniae ZJ05 (g) and S. typhimurium HYM2 (h). Values represent the averages of three independent experiments. i Three microliters of E. coli W3110 (pUC19-mcr-1) bacteria with different treatments (concentrations from 1 × 108 CFU/mL to 1 × 105 CFU/mL) were dropped on LB agar plates at 0, 3 and 12 h and cultured at 37 °C overnight. All the growth curves and bactericidal test were determined in triplicate, and representative data are displayed.

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