Fig. 1: The difference of cell-type composition and transcriptomic level between glioma and TDL. | Communications Biology

Fig. 1: The difference of cell-type composition and transcriptomic level between glioma and TDL.

From: Integrative and comparative single-cell analysis reveals transcriptomic difference between human tumefactive demyelinating lesion and glioma

Fig. 1

a Workflow diagram (Created with BioRender.com) showing the collection and processing of fresh samples from a high-grade glioma and a TDL for integrated analysis. b UMAP plot of cells from the two samples profiled in this study, with each color and number coded to indicate the associated cell types. c Feature plots of canonical markers for identify cell types after automatic annotation. d UMAP plot indicating the distribution of cells from TDL and glioma with corresponding color (left) and expression level of PTPRC in cells (right). e Proportion plot of cell-type composition in TDL. f Cell-type composition between TDL and glioma. g Comparison of proportion of immune clusters in TDL and glioma by nonparametric test (Mann–Whitney test). The data are expressed as median (interquartile range); n = 8 independent samples. A P-value < 0.05 was considered significant (*P < 0.05; **P < 0.01). h Volcano plot of differentially expressed genes (DEGs) that are upregulated (red) or downregulated (blue) in cells of TDL by comparing to cells of glioma. TDL tumefactive demyelinating lesion, QC quality control, UMAP Uniform Manifold Approximation and Projection, FC fold change.

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