Fig. 4: Transcriptomic difference of T cells between TDL and glioma and reclustering of T cells in TDL. | Communications Biology

Fig. 4: Transcriptomic difference of T cells between TDL and glioma and reclustering of T cells in TDL.

From: Integrative and comparative single-cell analysis reveals transcriptomic difference between human tumefactive demyelinating lesion and glioma

Fig. 4

a Volcano plot of differentially expressed genes (DEGs) that are upregulated (red) or downregulated (blue) in T cells of TDL by comparing to T cells of glioma. b UMAP plot of T cells from TDL after reclustering revealed 6 subclusters (C0, C1, C2, C3, C4, and C5). c Violin plots showing the specific expression distribution of selected genes related to T-cell subclusters. d Dotplot showing the characteristics of T-cell subclusters. e Dotplot of GO analysis (biological process) based on top 20 markers in C2. f Dotplot of GO analysis (biological process) based on top 20 markers in C5. g The pseudotime trajectory predicted by Monocle 3 and displayed by UMAP plot. Cells were ordered and colored by pseudotime in a gradient from purple to yellow. h The dynamic changes of expression level of selected genes along with pseudotime progression. TDL tumefactive demyelinating lesion, DEGs differentially expressed genes, FC fold change, GO Gene Ontology, UMAP Uniform Manifold Approximation and Projection.

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