Fig. 2: Increased viral replication depends on the targeted cell type but is independent of the ISG response.
From: Adaptation to host cell environment during experimental evolution of Zika virus
a Representative imaging of infectious foci at 48 h post-infection by parental virus versus serially passaged virus (i.e., passage 17) in Huh7.5.1 cells (left panels) and Vero cells (right panels) by immunostaining of E envelope proteins (red); nucleus stained by Hoechst (blue); scale-bar indicated. b Violin plot representation of the focus size in Huh7.5.1 cells and Vero cells at 48 h post-infection by parental virus versus serially passaged virus harvested at passage 17 of the viral passaging. The size index for each infectious focus is displayed by an individual dot; 5 independent measurements; p-values from pairwise comparisons using Wilcoxon rank sum test are shown. c–g Kinetic quantification in Huh7.5.1 (c), Vero cells (d), HEK-293 cells (e), U6A cells and STAT2 expressing U6A cells (f) and macrophages derived from monocytes (g) post-infection by parental versus serially passaged viral population obtained at passage 17 of the viral passaging. Results present the levels of intracellular ZIKV GE (upper panels) and MxA (lower panels) mRNA levels relative to the levels in non-infected cells, at the indicated time post-infection at MOI 0.1; 3-to-7 independent experiments; mean ± SD. The p-values of the statistical analysis of the kinetics are determined using a mixed linear model, which takes into account the levels at the time-points before those indicated in the table on the right side of the graphs. The indicated p-values are for the comparison of passaged viral population versus parental virus, when p < 0.05.
