Fig. 3: Deterministic and stochastic evolution of latent reaction norms.

Reaction norms of four morphological traits across three agar concentrations (0.5, 1.0, and 1.5%, x-axis in both panels). a Reaction norms across ancestral clones (black circles and lines) and treatment-level average trait values (CTT-HA and CTT-SA evolved populations, dark and light blue circles and lines, respectively). n = 3 independent replicates (gray circles). b Reaction norms for each individual Anc sub-clone (c1–c6) and each TS-abiotic evolved population across the three biological replicates (n = 3). Labels at the end of each reaction norm indicate the identity of the ancestral clone or evolved population. In both a and b, large circles and error bars indicate the mean ± SEM, respectively. (Significance of variables’ contribution and their interaction were calculated for both a and b with two-way ANOVA. Employed models and results of all comparisons are reported in Data Fig. 3).