Fig. 2: Suppression of RUNX1 induces apoptosis and cell cycle arrest in glioblastoma cells. | Communications Biology

Fig. 2: Suppression of RUNX1 induces apoptosis and cell cycle arrest in glioblastoma cells.

From: A RUNX-targeted gene switch-off approach modulates the BIRC5/PIF1-p21 pathway and reduces glioblastoma growth in mice

Fig. 2

a Immunoblotting of each GBM cell line transduced with control or RUNX1 shRNAs. Control (sh_Luc) and sh_RUNX1 cells were cultured in the presence of 5 μM of doxycycline. b Growth curves of A172, KALS-1, LN229 and T98G cells transduced with control (sh_Luc) or with RUNX1 shRNAs (#1 and #2) in the presence of 5 μM of doxycycline. n = 3. c RUNX1 depression-induced apoptosis. Nondepleted and RUNX1-depressed A172 and LN229 were treated in the presence of 5 μM of doxycycline for 6 days, KALS-1 and T98G were treated for 4 days. The line in the centre of the rhombus indicates the mean value, and the vertical width indicates the 95% CI. n = 3. d RUNX1 depression induced cell cycle arrest. G2/M DNA content of cells was increased. Nondepleted and RUNX1-depressed cells were treated in the presence of 5 μM of doxycycline for 4 days. Cells were analysed by flow cytometry. n = 3. Data represent mean ± 95% CI. *p < 0.05, **p < 0.01, ***p < 0.001, by two-tailed Student’s t-test.

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