Table 1 Thermodynamic parameters of the interaction between Ga-substituted Fd and PSI trimer.

From: Structure of cyanobacterial photosystem I complexed with ferredoxin at 1.97 Å resolution

 

1st measurement

2nd measurement

3rd measurement

Mean ± S.E.M.

Kd (nM)

703

994

577

758.0 ± 123.5

ΔG (kcal/mol)

−8.4

−8.2

−8.5

−8.4 ± 0.1

ΔH (kcal/mol)

1.0

1.1

1.2

1.1 ± 0.1

−TΔS (kcal/mol)

−9.4

−9.3

−9.7

−9.5 ± 0.1

n

2.9

3.2

2.5

2.9 ± 0.2

  1. Three independent ITC experiments were performed at 25 °C. Each set of the thermodynamic parameters obtained by fitting normalized ΔH in the binding isotherm are shown without fit errors. Mean and S.E.M. values were also presented, indicating the averaged value and the standard error of the mean, respectively.
  2. Kd dissociation constant, ΔG change in Gibbs free energy, ΔH change in enthalpy energy, ΔS change in entropy, n binding stoichiometry.
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