Fig. 6: PA promotes HFD-induced intestinal impairments.

Mice were divided into four groups: the Con, LDR (0.05 Gy*10) +PA, HFD, and HFD + PA groups. a Trial design for exploration of the effects of PA on mice. b Representative electron microscopy images of intestinal structure among the four groups. 20000X. c Relative FITC-dextran levels. d Macroscopic scores. e mRNA expression of the intestinal tight junction genesZO-1, Occludin, Claudin-1 and Claudin-2 among the four groups. f Immunohistochemical detection of Occludin and ZO-1 in intestinal tissues. Scale bar: 100 µm. g The HCT116 cell line was used to detect theeffects of PA on intestinal tight junction gene mRNA expression at the indicated PA concentrations. h The HCT116 cell line was used to detect the effects of PA on intestinal tight junction gene mRNA expression at the indicated PA treatment timepoints. The data are the means ± SDs. The Mann-Whitney test or two-tailed unpaired Student’s t-test was used for statistical analyses. *p < 0.05 indicates a significant difference. Error bars indicate SEM (standard error of the mean). a was created by Ruixue Huang and the elements used was permitted by Biorender (https://biorender.com/) because Ruixue Huang is one of the registered user.