Fig. 5: Tumors containing scIgGs had an overall reduced immune cytokine levels.
From: Impairment of IgG Fc functions promotes tumor progression and suppresses NK cell antitumor actions
a Heat map of cytokine /chemokines levels detected in tumor lysates of PyMT+/scIgG+ vs PyMT+, n = 4. Tumor lysates were used for profiling a panel of 62 cytokines and chemokines (RayBio, c3 series, 62 cytokines) and tumor lysates used in each array were normalized with protein concentrations. b Heat map of cytokines/chemokines detected in tumor lysates of BT474-IdeS vs BT474. n = 6. Tumors with scIgGs had reduced cytokines more than 40% of the 62 panel RPPA (red color at X-axis) when compared to that in the control tumors. Fold of change (Y-axis) indicates ratio of cytokine levels in (c) PyMT+/scIgG+/PyMT+ and in (d) BT474-IdeS/ BT474. e, f Tumors with scIgGs had significantly lower IFN-γ levels in comparison with the control lysates. The levels of IFN-γ were analyzed using RPPA array data for (e) PyMT+/scIgG+ vs PyMT+ pair (n = 4, p < 0.01) and (f) BT474-Ides vs BT474 tumors pair comparison (n = 6, p = 0.0767). g, h Tumors with scIgGs had significantly lower TNF-α levels in comparison with the control PyMT+ tumor lysates. Relative levels of TNF-α were shown based on RPPA array analysis, (g) for PyMT+/scIgG+ vs PyMT+ pair (n = 4, p < 0.001) and (h) for BT474-IdeS vs BT474 (n = 6, p < 0.05).
