Fig. 1: Characterization of S- and W-EGFP cells by scRNA-seq.
From: Tmsb10 triggers fetal Leydig differentiation by suppressing the RAS/ERK pathway

a High-quality scRNA-seq datasets obtained from 80 S-EGFP and 341 W-EGFP cells were analyzed by hierarchical clustering. As indicated by the dendrogram (upper), the cells were divided into five cell clusters: cluster A (204 W-EGFP cells; light blue), cluster B (80 W-EGFP cells; blue), cluster C (57 W-EGFP cells; orange), cluster D (13 S-EGFP cells; green), and cluster E (67 S-EGFP cells; red). The heatmap (lower) was based on genes in clusters C, D, and/or E whose expressions were altered. Genes differentially expressed in these clusters (group I to III) are shown. Tmsb10 in group III is highlighted in red. b, c Results of t-SNE b and Monocle pseudo-time trajectory analyses c of the datasets are shown. The cellular distribution is shown (each dot represents one cell), with colors labeling the five clusters of cells as in a. d–g Expression levels of Cyp11a1 d, Calr e, Tmsb10 f, and Arx g in the cells above, as determined by scRNA-seq. Each dot represents one cell.