Fig. 2: Increased expression of Tmsb10 in putative FLC progenitors.
From: Tmsb10 triggers fetal Leydig differentiation by suppressing the RAS/ERK pathway

a The testes of FLE-EGFP;Tmsb10-mCherry mouse fetuses at E16.5 were analyzed by immunofluorescence. Representative images of staining with EGFP (green), mCherry (red), and LAMININ (white), with all three stains overlaid in the image on the right. Arrows indicate cells double positive for mCherry and LAMININ. Closed arrowheads indicate cells double positive for mCherry (weakly stained) and EGFP. Open arrowheads indicate FLCs strongly stained with EGFP. Asterisks mark testis tubules. Scale bar = 20 µm. b–d Expressions of EGFP b, Tmsb10 c, and Pecam1 d in N-EGFP (EGFP-negative, N, open bars), W-EGFP (W, light blue bars), and S-EGFP (S, blue bars) cells prepared from FLE-EGFP fetal testes at E16.5. The data were normalized by Rn18s and are presented as means ± SEM. Letters a, b, and c denote significant differences between the cell groups, N-EGFP (N), W-EGFP (W), and S-EGFP (S). n = 3. p < 0.01.