Table 2 Components of the capacitating medium.

From: Predicting fertility from sperm motility landscapes

Component

Quantity (grams)

Hepes (buffer)

0.1431 g

NaCl

0.1965 g

KCl

0.0069 g

Glucose

0.0270 g

Na2HPO4

0.0033 g

MgSO4

0.0030 g

CaCl2

0.0198 g

Sodium lactate

0.0729 g

Sodium pyruvate

0.0033 g

Bovine serum albumin

0.1452 g

Sodium bicarbonate (15 mM)

0.0378 g

  1. The represented amounts are calculated to prepare 30 mL of medium. All reagents were purchased from Sigma Aldrich (Saint Louis, Missouri, USA).
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