Fig. 3: Genetically modified tobacco and dsPsFAR expression in transformants.
a Schematic diagram of the dsPsFAR expression cassette in the pCAMBIA1301-dsPsFAR vector introduced into Nicotiana tabacum cv. Petit Havana via Agrobacterium-mediated transformation. Two arrows in the opposite direction, respectively, indicate sense and antisense strands of dsPsFAR. HYG: hygromycin; NOS: Nos terminator; GUS: β-glucuronidase. b Tobacco leaf discs were pre-incubated in medium with kanamycin and then transferred to medium for differentiation and rooting. Seedlings were transferred into nutrient soil and grown in a greenhouse to obtain the T1 generation. WT: wild-type plants, Transgenic: transgenic plants. c Selection of positive tobacco transformants via PCR amplification of the hpt gene. The first lane is marker (M), followed by 26 transformants (#1–#26), blank control (B), negative control (N), and Positive control (P). d Relative expression level of dsPsFAR in nine randomly selected T0 GM tobacco. Data were normalized and are represented as the mean ± SEM from three biological replicates. The different letters indicate significant differences determined by one-way ANOVA followed by the Tukey’s multiple comparison test (P  <  0.05).
