Fig. 4: RNAi effects, lethality, and survival rate of P. solenopsis microinjected with dsRNA or fed on dsRNA expressing GM tobacco. | Communications Biology

Fig. 4: RNAi effects, lethality, and survival rate of P. solenopsis microinjected with dsRNA or fed on dsRNA expressing GM tobacco.

From: Fatty acyl-CoA reductase influences wax biosynthesis in the cotton mealybug, Phenacoccus solenopsis Tinsley

Fig. 4

a Validation of RNAi effects of dsRNA microinjection (left) and GM tobacco feeding (right) using RT-qPCR. Total RNA for PsFAR was extracted from cotton mealybugs 3 days post injection (n = 7) and after feeding on GM tobacco for 5 days (n = 10). b Lethality in treated insects: dsGFP and WT tobacco-treated cotton mealybugs were normal and covered with a heavy waxy layer around their body surface, while dsPsFAR treated and GM tobacco treated insects exhibited abnormal coloration, reduced wax secretion, and unsuccessful molting. c Dynamic analysis of the survival rate of P. solenopsis after dsRNA injection (left) and feeding on tobacco plants (right). WT: insects fed on wild-type plants, Transgenic: insects fed on GM tobacco plants. dsGFP and WT were respectively set as negative controls, n = 30 insects. Means ± SEM were calculated with results from three biological replicates. Asterisks denote a significant difference as determined by Student’s t-test; *P < 0.05, **P < 0.01.

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