Fig. 3: Analysis of pluripotency-related gene expression in mouse, chick, Okinawa rail, Japanese ptarmigan, and Blakiston’s fish owl-derived iPSCs and parent fibroblasts.
From: Induced pluripotent stem cells of endangered avian species
a mRNA expression levels of POU5 (mammalian Oct3/4), Nanog, Sox family, and Lin28 in mouse, chicken, Okinawa rail, Japanese ptarmigan, and Blakiston’s fish owl-derived iPSCs and fibroblasts. endo-POU5 (mammalian Oct3/4) (i), endo-Sox2 (ii), endo-Nanog (iii), Sox3 (iv), endo-Lin28a (v), and endo-Lin28b (vi) represents. b Pluripotency-associated genes commonly expressed in mouse, chick Okinawa rail, Japanese ptarmigan, and Blakiston fish owl iPSCs. Cdh1 (i), Fbxo15 (ii), Esrrb (iii), Sall4 (iv), Dnmt3b (v), Nanog-like (vi), and Rex1 (vii) represents. c Pluripotency-associated genes differentially expressed in mouse, chick Okinawa rail, Japanese ptarmigan, and Blakiston fish owl iPSCs. Endo-Klf4 (i), Endo-c-Myc (ii), Tfcp2l1 (iii), Tbx3 (iv), Endo-Klf2 (v), and Prdm14 (vi) represents. d mRNA expression levels of primordial germ cell (PGC) marker genes. Vasa (i), Dazl (ii) represents. Gene expression was quantified relative to the GAPDH internal control. The fibroblast expression level was 1.0. Blue bars, mouse; red bars, chicken; yellow bars, Okinawa rail; green bars, ptarmigan; and purple bars, Blakiston’s fish owl-derived cells. Mouse and chicken: Fibroblasts and the iPSCs (passage number 3; P3) represents. Okinawa rail, Japanese ptarmigan, and Blakiston’s fish owl: Fibroblasts, iPSCs (P3), and iPSCs (P10) represents. Centerlines of box plots indicate medians; box limits indicate the 25th and 75th percentiles. n = 4 (Chicken fibroblast in Pou5, Sox2, Nanog, Lin28a, Lin28b, Cdh1, Fbxo15, Esrrb, Klf4, Tbx3, Klf2, Prdm14, Vasa, and Dazl. Okinawa rail fibroblast in Sox2, Esrrb, Klf4, Tbx3, and Klf2.), n = 6 (other). *P < 0.05, **P < 0.01.
