Fig. 7: MMV688754 does not inhibit TaPin1 activity.
a Chemical structures of DTM (Pin1 inhibitor) and the test compound MMV688754. b Purification of recombinant His-tagged TaPin1 protein. c Inhibition assay showing that DTM alone inhibited TaPin1 prolyl-isomerase activity in vitro. TaPin1 (10 µM) was incubated for 24 h at 4 °C degrees with 500 µM DTM, MMV688754 or DMSO. Mixtures were diluted 1000-fold, and TaPin1 activity was assayed for 1 min by reading the absorbance at 380 nm. Apparent velocity constants (kobs) were extrapolated from these curves. While DTM effectively inhibited TaPin1 activity, MMV688754 did not have any inhibitory effect. These results are representative of three independent experiments, which are shown quantitatively below. Mean values ± SD. n = 3 independent experiments. One-way Anova statistical test followed by Dunnett’s multiple comparison test: ****p < 0.0001.
