Fig. 3: B56γ is required for efficient tumor cell migration and invasion. | Communications Biology

Fig. 3: B56γ is required for efficient tumor cell migration and invasion.

From: A functional interaction between liprin-α1 and B56γ regulatory subunit of protein phosphatase 2A supports tumor cell motility

Fig. 3

a Silencing of either B56γ or liprin-α1 inhibits MatrigelTM invasion by MDA-MB-231 cells. Top, fields from wells with invading cells. Bottom: left, immunoblotting from lysates of siRNA-transfected cells used for invasion (50 µg protein/lane); right: bars represent the average number of invading cells per field; n = 64–71 fields from 10 to 12 wells, from 4 experiments; n = 24 fields from 4 wells for control (no stimulus). b Silencing of either B56γ or liprin-α1 inhibits tumor cell migration. Top, frames from time-lapses of GFP-positive MDA-MB-231 cells transfected with siRNAs. Bottom: left, immunoblotting from lysates of cells used for random migration (50 µg protein/lane). Right: average speed and directionality of migrating cells; n = 139–167cells/experimental condition from 2 experiments. c Silencing of either B56γ or liprin-α1 inhibits lamellipodia dynamics. Top, frames from time-lapses of GFP-positive MDA-MB-231 cells from experiments shown in b. Bottom: average persistence of lamellipodia (left; n = 22 cells, 137–211 lamellipodia analyzed from 2 experiments) and frequency (right; n = 22, from 2 experiments). Graph bars: mean and SE.

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