Fig. 1: Induced double knockout of Msi1 and Msi2 in photoreceptor cells. | Communications Biology

Fig. 1: Induced double knockout of Msi1 and Msi2 in photoreceptor cells.

From: The Musashi proteins direct post-transcriptional control of protein expression and alternate exon splicing in vertebrate photoreceptors

Fig. 1

a Immunofluorescence micrographs of retinal cross-sections collected 14 days after tamoxifen injection at postnatal day 30 from Msi1+/+/Msi2+/+ littermate control and Msi1−/−/Msi2−/−, stained for MSI1(green), MSI2 (magenta), and DAPI (blue). ONL outer nuclear layer (photoreceptor cell layer). The brackets on the left indicate the layer of photoreceptor cells from which the genes are being deleted. IS: inner segment (photoreceptor cell layer). ONL outer nuclear layer (photoreceptor cell layer), INL inner nuclear layer, GC ganglion cell layer. Objective, 40x. b Immunoblot of lysates from Msi1+/+/Msi2 +/+ and Msi1−/− /Msi2−/− retinas collected between 0 and 70 days after tamoxifen injections at postnatal day 30 and probed with antibodies to MSI1, MSI2 and TUBB (β-tubulin, loading control). Size markers indicate the molecular weight in kDA. See Supplementary Fig. 1 for uncropped blots.

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