Fig. 4: Normal photoreceptor response to light and retinal morphology in the single knockouts Msi1 or Msi2 in photoreceptor cells.

a Scotopic mean A-wave response from single Msi1 ^−/− (left) or Msi2 ^−/− (right) knockouts in photoreceptors (red lines) and littermate controls (black lines) recorded between 0 and 230 days post-tamoxifen injection. Scotopic waveforms were obtained using 0.151 cd-s/m² flashes. The insets of panels A and B show representative scotopic (dark-adapted) electroretinograms from a single knockout (red) or control (black) retina at D230 post-injection using 0.151 cd-s/m² flashes. Scotopic waveforms were obtained after overnight dark adaptation using 0.151 cd-s/m² flashes. b Representative H&E-stained sections from retinas of single Msi1 ^−/− (left) or Msi2 ^−/− (right) knockouts in photoreceptor cells and littermate controls collected 230 days post-tamoxifen injection. ONL: outer nuclear layer (Photoreceptor nuclei), INL: inner nuclear layer, GC: ganglion cells. 40X objectives, scale bar, represents 10 μm. c Spider plots of ONL thickness for single Msi1 ^−/− (left) or Msi2 ^−/− (right) knockouts (red) and littermate controls (black). The plots display the thickness of the ONL as the number of nuclei measured at ten points stepped by 400 µm from the optical nerve in retinas collected 230 days after tamoxifen injection. Data are shown as mean ± SEM of 8 eyes, from four animals. Supplementary Data 15 contains the source data underlying the graphs.