Fig. 2: Comparison of apparent affinities of dimeric and tetrameric peptides in ITC, TRIC, BLI and FPS and target protein consumption.
From: Multivalent binding kinetics resolved by fluorescence proximity sensing
a Apparent binding affinities of seven benchmark peptides (four dimeric and three tetrameric) were measured using FPS (dynamic KD value from n = 1 measurements of 3 different concentrations), BLI (steady-state KD value from n=1 measurements at seven different concentrations), ITC (KD value from n = 3 and n = 2 measurements) and TRIC (Ki values from n = 2 measurements). For complete measurements, see Supplementary Fig. 5, 6, 7 and 10 respectively. b Amount of target protein consumed for affinity determination of one peptide in the four methods tested (FPS: one sensor chip functionalization, BLI: functionalization of eight sensors, ITC: one run with 16 injections, TRIC: 16-point dose response in displacement assay setup). Source data are provided in Supplementary Data 1.
