Fig. 4: D-Flow exposure causes elevated expression of pro-inflammatory ICAM1 in EC.
From: Angular difference in human coronary artery governs endothelial cell structure and function
a–c Immunofluorescence staining of EA.hy926 for ICAM1 (n = 3) after 4 h fluid flow exposure, using human coronary microchannels with 30° (a), 60° (b) and 80° (c) branching points. DAPI staining is shown in blue. ICAM1 fluorescence signal in individual EA.hy926 cells (dots) from three individual experiments. Fluorescence intensity AU values per individual cells are indicated together with the mean. Total number of cells, n ≥ 45. Values represent the mean ± SD. *p < 0.05, **p < 0.01 and ***p < 0.001, by one-way ANOVA. Magnification: ×20, Scale: 100 µm. d Tile scan images microchannels harbouring EC stained for ICAM1. Magnification: ×10, Scale: 2000 µm.
