Fig. 6: Increasing bifurcation angles of coronary artery incrementally impacts the EC morphology.
From: Angular difference in human coronary artery governs endothelial cell structure and function
FE-SEM (top) based structural and surface level analysis of EC exposed to D-Flow using 30°, 60° and 80° microchannels. Magnification: ×5000, Scale: 10 µm. Flow exposed (4 h; 30°, 60° and 80° microchannels) EA.hy926 cells stained for cell-cell contact markers CD31 (second) and CD144 (penultimate). DAPI staining is shown in blue. Circularity of such cells was assessed using ImageJ. Cell circularity AU values per individual cells are indicated together with mean. Total number of cells, n ≥ 30. Values represent the mean ± SD. *p < 0.05, **p < 0.01 and ***p < 0.001, by one-way ANOVA. Magnification: ×50/×63, Scale: 80 µm/20 µm. Cell cytoskeletal F-actin (bottom) staining for EA.hy926 exposed to D-Flow (D-variable or D-fixed regions) in 30°, 60° and 80° microchannels, using phalloidin. DAPI staining is shown in blue. Magnification: ×63, Scale: 20 µm.
