Fig. 2: Targeted expression in OBCs after intravitreal injection. | Communications Biology

Fig. 2: Targeted expression in OBCs after intravitreal injection.

From: Bipolar cell targeted optogenetic gene therapy restores parallel retinal signaling and high-level vision in the degenerated retina

Fig. 2

a A representative rd1 retina four weeks after intravitreal injection of Mela(CTmGluR6)-IRES-TurboFP635. Inserts show higher magnification scanning micrographs taken from the bipolar cell layer at specified regions, with relatively high (1) or low (2) transduction. Despite the highlighted heterogeneity, OBCs were transduced across the retina. b Expression was specific for OBCs in the rd1 retina (TurboFP635 in green; Gαo in red; DAPI in blue). c A Mela(CTmGluR6)-mKate fusion protein demonstrates robust targeted expression to the OBC dendrites (mKate in green; Gαo in red; DAPI in blue). Scale bars 500 µm in (a) and 50 µm in other panels.

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