Fig. 1: Global pTyr is reduced in cardiac hypertrophy associated with ErbB2 overexpression or myosin heavy chain mutation. | Communications Biology

Fig. 1: Global pTyr is reduced in cardiac hypertrophy associated with ErbB2 overexpression or myosin heavy chain mutation.

From: Alteration in tyrosine phosphorylation of cardiac proteome and EGFR pathway contribute to hypertrophic cardiomyopathy

Fig. 1

a Western Blots show a decrease in pTyr on TgErbB2 (p = 0.0298) mice and R403Q-α-MyHC (p = 0.003) heart homogenates. The Troponin I signal shows corresponding total protein loading. b Densitometry analysis shows that after the pTyrosine signal was normalized to total cTnI, the TgErbB2 and R403Q-α-MyHC mice significantly decreased in pTyr. c Western Blots show expression levels of total Src and p-Src (Tyr 416) and GAPDH as a loading control. d Densitometry analysis revealed that Src levels are significantly elevated in R403Q-α-MyHC (p = 0.0282), whereas e and f Show that p-Src phosphorylation levels are elevated in R403Q-α-MyHC but did not reach statistical significance. Signals in d and e were normalized to total GAPDH, whereas in f pSRC was normalized to total SRC. Ntg (n = 3), TgErbB2 (n = 3), and R403Q-α-MyHC (n = 3), error bars represent S.E.M. One-way ANOVA was used to compare groups.

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