Fig. 1: Diabetic db/db mice develop more severe acute pancreatitis.

a Glucose tolerance of db/db mice at 8 and 24 weeks old, n = 7–10. p < 0.001 for the entire time course. b, c White blood cell count and different white blood cell count, n = 11–28. d, e Serum amylase activity and serum lipase activity in mice with induced acute pancreatitis followed by injection of BMCs from db/db mice, n = 10. f–h The severity was assessed on H&E-stained sections by measuring inflammatory cell infiltration, acinar cell necrosis and vacuolization, and edema of the pancreas, scale bar = 100 μm or 200 μm, respectively. The total severity score is calculated as the sum of inflammation, necrosis, vacuolization, and edema, n = 10. i, j Paraffin sections were stained with an antibody against MPO. Representative images of MPO-stained sections in db/db mice are shown. Black arrows indicate MPO⁺ cells, scale bar = 100 μm. The number of MPO⁺ cells per microscopy field was estimated by ImageJ, n = 10. MPO, myeloperoxidase. Unpaired, 2-tailed Student’s t test for data with normal distribution. Non-parametric Mann–Whitney analysis was used for the data did not follow normal distribution.