Fig. 3: Increased purine metabolites in GMP cells of db/db mice aged at 24 weeks old. | Communications Biology

Fig. 3: Increased purine metabolites in GMP cells of db/db mice aged at 24 weeks old.

From: The purine metabolite inosine monophosphate accelerates myelopoiesis and acute pancreatitis progression

Fig. 3

The metabolic features of FACS-sorted GMP cells were detected by targeted metabonomics in db/db mice at the age of 8 and 24 weeks. a The scheme of the study design. b Volcano blot showing the difference of metabolite between two groups, n = 6–16. The pink line represented where p value was 0.05. c–e Intracellular levels of ribose-5-phosphate, guanosine triphosphate, and inosine monophosphate in GMP cells of db/db mice. n = 6–14. f–h The mRNA expressions of ADSS, GMPR, and IMPDH2 were analyzed by ddPCR. n = 6. i Representative of ddPCR results using FACS-sorted GMP cells. ADSS, adenylosuccinate synthetase. Unpaired, 2-tailed Student’s t test for data with normal distribution. Non-parametric Mann–Whitney analysis was used for the data did not follow normal distribution. GMPR, guanosine monophosphate reductase. IMPDH2, IMP dehydrogenase 2.

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