Fig. 5: Inosine monophosphate treatment promotes mitochondrial transcription. | Communications Biology

Fig. 5: Inosine monophosphate treatment promotes mitochondrial transcription.

From: The purine metabolite inosine monophosphate accelerates myelopoiesis and acute pancreatitis progression

Fig. 5

a RT–QPCR analysis of the mRNA expression of NRF1 and mtTFA in lineage−/low cells after treated with IMP for 24 h, n =  6. b–e Western blot analysis expression level of NRF1, mtTFA and PFAS in IMP-treated cells for 24 or 72 h compared with controls, n =  15–27. Data are presented as the means ± SEM. f–h ADSS, ATPase and IMPDH2 activity in lineage−/low cells which were stimulated with IMP for 0, 4, and 8 h, n = 6. i ATP and GTP production in lineage−/low cells after 24 and 72 h of IMP treatment, n = 6. j, k Oxygen consumption rate analysis of the Lineage−/low cells which were exposed to PBS or 15 mM IMP for 6 h, n = 6. One-way ANOVA with Dunnett was used to compare groups against control. IMP Inosine-5′-monophosphate, ADSS adenylosuccinate synthetase, IMPDH2 Inosine-5′-monophosphate dehydrogenase 2, ATP Adenosine triphosphate, GTP guanosine triphosphate, NRF1 nuclear respiratory factor 1, PFAS phosphoribosylformylglycinamidine synthase.

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