Fig. 1: Blue-light-induced conformational change of CraCRY determined using smFRET. | Communications Biology

Fig. 1: Blue-light-induced conformational change of CraCRY determined using smFRET.

From: Direct experimental observation of blue-light-induced conformational change and intermolecular interactions of cryptochrome

Fig. 1

a Single-molecule fluorescence time traces show the donor and acceptor fluorescence intensity with donor excitation under dark or lit conditions (upper panels). Single-molecule FRET time traces show the relative FRET efficiency (lower panels). b CraCRY is labeled with Atto550 (green)/Atto647 (orange) as the donor and acceptor at E595C or C317 sites, respectively. The PHR and CTE domains are shown in gray and blue, respectively. The predicted structure of CraCRY is shown to illustrate the labeled sites. c smFRET data converted into distance histograms of CraCRY molecules under dark or lit conditions. The normalized probability distribution functions (pdfs, shown in bars) are fitted with Gaussian distributions (solid lines).

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