Table 3 Differentiation medium composition for tumor progression organoids and patient-derived organoids (PDOs).

0.125 nM Wnt surrogate FC fusion protein (U-protein express BV, #N001)

x

–

–

–

–

–

R-spondin-CM (homemade)

10% v/v

–

–

–

–

5% v/v

10% v/v Noggin-CM (homemade)

x

x

x

x

–

–

1x B27 (Fisher Scientific, #15360284)

x

x

x

x

x

–

1.25 mM N-acetyl-cysteine (Sigma, #N0636)

x

x

x

x

x

–

500 nM A83 (Biotechne, #2939))

x

x

x

x

x

–

50 ng/mL EGF (Peprotech, #AF-100-15)

x

–

x

–

–

–