Fig. 3: Repair pathway of nicked phosphate backbone.
From: Information decay and enzymatic information recovery for DNA data storage
a Quantification of repair potential on aged model DNA (4 weeks, 30 °C), with three enzymes (APE1, Bst Polymerase, Taq ligase), compared to repair results when leaving out either one of the three enzymes during the repair procedure; s.d. from three qPCR replicates, DNA concentration normalized by the nonrepaired sample (rel. conc. of nonrepaired sample = 1.0). b Suggested repair pathway of a nick: when the phosphate backbone has been cleaved by β-elimination, APE1 can ingest the 3’-phospho-α,β-unsaturated aldehyde and render the DNA strand with a 3’-OH terminus such that a polymerase can elongate the backbone and insert the missing base. The taq ligase will then seal the strand.
