Fig. 1: Characterization of the intron lariat debranching enzyme FgDbr1.

a Phylogenetic tree of Dbr1 homologues. Amino acid sequences were aligned using the ClustalW, and MEGA X software was used to perform phylogenetic analysis using the neighbor-joining method with 1000 bootstrap replicates. Fg Fusarium graminearum, Fo Fusarium oxysporum, Mo Magnaporthe oryzae, Nc Neurospora crassa, Sp Schizosaccharomyces pombe, Cn Cryptococcus neoformans, Sc Saccharomyces cerevisiae, Ce Caenorhabditis elegans, Hs Homo sapiens, At Arabidopsis thaliana. b Domain architecture of FgDbr1. MPE domain including LRL motif is conserved in FgDbr1. MPE methallophosphoesterase domain, LRL lariat recognition loop, CTD C-terminal domain. c Heterologous expression of FgDBR1 in yeast. Relative levels of intron lariat were quantified by quantitative real-time PCR (qRT-PCR) in the mutant containing empty vector pYES2 or pYES2-FgDBR1. Relative level of intron lariat in the wild-type BY4741 containing empty vector pYES2 was not detectable. Error bars indicated standard deviations of three biological replicates. ACT1, RPL28 and RPS17A represent introns of ACT1, RPL28 and RPS17A genes in S. cerevisiae, respectively. d Subcellular localization of FgDbr1. FgDbr1 was fused with green fluorescent protein (GFP) and histone H1 with red fluorescent protein (RFP). The yellow color in the merged images indicates co-localization. Scale bar = 10 μm.