Fig. 4: Transcriptome analysis of RNase R- treated or untreated samples in F. graminearum strains.

a Workflow of intron lariat enrichment for RNA sequencing (RNA-seq). Total RNA was extracted from each strain grown for 24 h in liquid CM and digested with RNase R for linear RNA degradation. The remaining RNAs were further subjected to rRNA depletion and subsequently used for RNA-seq library construction. b Distribution of mapped read counts originating from exonic and intronic regions. c Verification of the elimination of linear RNA by RNase R. Relative transcript levels of the wild-type and ΔFgdbr1 samples with or without RNase R treatment were determined by qRT-PCR analysis. Error bars represent standard deviations of three biological replicates.