Fig. 5: Transcript and intronic expression signatures in ΔFgdbr1 mutant. | Communications Biology

Fig. 5: Transcript and intronic expression signatures in ΔFgdbr1 mutant.

From: Intron turnover is essential to the development and pathogenicity of the plant pathogenic fungus Fusarium graminearum

Fig. 5

a Gene ontology (GO) enrichment analysis of the upregulated genes in the ΔFgdbr1 mutant. b Transcript levels of ten selected genes related to ribosome and translation in the F. graminearum strains. Transcript levels of selected genes were quantified by qRT-PCR. c Relative amount of ribosomal RNAs (rRNAs) in the wild-type and ΔFgdbr1 strains. 18 S, 28 S and pre-rRNA levels were quantified by qRT-PCR. Error bars represent standard deviations of three biological replicates. **p < 0.01; ***p < 0.001. d List of genes containing top 10 enriched intron lariat in ΔFgdbr1 mutant compared to wild type. e The proportion of genes with the intron lariat fold changes in three categories. Relative intron lariat levels of total genes or upregulated genes corresponding GO term ‘ribosome (GO:0005840) were analyzed based on RNA-set data of each strain.

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