Fig. 7: Identification of intron lariats in F. graminearum.
a Genome browser images of four selected ribosomal protein genes. Aligned RNA-seq data generated from RNase R treated or untreated samples of F. graminearum strains were visualized using IGV. The y-axis represents the TPM. Thick box, coding sequence; thin box, untranslated region; line, intron. b Schematic representation of divergent primers used for amplifying intron lariat RNAs. Green and blue box, exon; line, intron; A, branch point. c Detection of intron lariat RNAs using reverse transcription PCR (RT-PCR) assays. RNA samples of each strain with or without RNase R treatment were amplified using divergent primers described in a. The images were obtained from 30 cycle of PCR using 2% agarose gels. d Verification of RNase R treatment. Relative intron lariat levels of ĪFgdbr1 samples with or without RNase R treatment were determined by qRT-PCR analysis. e Relative intron lariat levels in the wild-type and ĪFgdbr1 strains. Intron lariat RNA levels of RNase R treated samples were quantified by qRT-PCR. Error bars represent standard deviations of three biological replicates. ***pā<ā0.001.
