Fig. 9: Reduced protein translation rate in the ΔFgdbr1 strains.

a Mycelial growth of F. graminearum strains on CM and CM supplemented with hygromycin B. b Mycelial growth of F. graminearum strains on CM and CM supplemented with cycloheximide. The photographs were taken 5 days after inoculation. c Inhibition rate of mycelial growth in response to protein synthesis inhibitors. The percentage of mycelial growth inhibition was examined 5 days after inoculation on CM with protein synthesis inhibitors. Error bars represent standard deviations of three biological replicates. ***p < 0.001. d Visualization of newly synthesized proteins in germinating conidia of F. graminearum strains. Click-iT protein synthesis assay kit was used to visualize protein translation. Conidia of each strain were inoculated in liquid CM with or without translation inhibitor and processed for click reaction. The newly synthesized proteins were examined under a fluorescence microscope using the filter L5. Scale bar = 25 μm. e Quantification of the mean fluorescent intensity of each sample. The mean GFP fluorescence intensity in a transverse section of individual conidium, indicating newly synthesized proteins, was measured using LAS x software. A total of 100 conidia was observed in each sample. Lower and upper whiskers represent 1.5 times of the lower and upper interquartile ranges (IQR), respectively, and median values were indicated by solid black lines. Same letters mean there is no significant difference between strains, different letters indicate statistically significant differences with p < 0.01.