Table 2 Precision of MOP phosphorylation assays.

From: A bead-based GPCR phosphorylation immunoassay for high-throughput ligand profiling and GRK inhibitor screening

7TM Phosphorylation Assay

% CV Interassay

% CV Intra-assay

Z′ factor

pT370-MOP

9.8

9.3

0.87

pS375-MOP

15.1

5.5

0.69

pT376-MOP

11.9

9.5

0.80

pT379-MOP

12.1

8.3

0.82

np-MOP

9.0

6.5

N.a.a

  1. Values were calculated from at least 30 determinations performed in duplicate.
  2. aNot applicable.
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